Electroporation optimization to deliver plasmid DNA into dental follicle cells
نویسندگان
چکیده
منابع مشابه
Spatially and temporally controlled gene transfer by electroporation into adherent cells on plasmid DNA-loaded electrodes.
Functional characterization of human genes is one of the most challenging tasks in current genomics. Owing to a large number of newly discovered genes, high-throughput methodologies are greatly needed to express in parallel each gene in living cells. To develop a method that allows efficient transfection of plasmids into adherent cells in spatial- and temporal-specific manners, we studied elect...
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The dental follicle is an ectomesenchymal tissue surrounding the developing tooth germ. Human dental follicle cells (hDFCs) have the capacity to commit to differentiation into multiple cell types. Here we investigated the capacity of hDFCs to differentiate into neural cells and the efficiency of a two-step strategy involving floating neurosphere-like bodies for neural differentiation. Undiffere...
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Loree Heller and M. Lee Lucas Center for Molecular Delivery, University of South Florida, Tampa, FL 33612, Department of Medical Microbiology, University of South Florida, Tampa, FL 33612 __________________________________________________________________________________ * Correspondence : Loree Heller, University of South Florida, Center for Molecular Delivery, MDC16, 12901 Bruce B. Downs Blvd....
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ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2009
ISSN: 1860-6768,1860-7314
DOI: 10.1002/biot.200900039